J. Cell Biol. 2011;193(5):917–933.
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CYTOOplate™ 96 RW Custom FN
CYTOO offer custom micropattern designs to meet your requirements.
The CYTOOplates Custom are available in 96well standard SBS format with custom micropatterns and adhesive proteins suitable for various applications and assays. Whether you are looking for a specific micropattern shape, size or 96well layout, CYTOO can manufacture it for you.
The minimum quantity for ordering CYTOOplates Custom is a set of 10 microplates (in the 96well format) and can be purchased coated in fibronectin (fluorescently labeled or not), or activated surfaces.
CYTOO Custom micropattern designs allow you to:
• work with our standard pattern geometries but making them smaller*, larger or at different densities (pattern pitch)
• mix different micropattern shapes in the same experiment or condition
• tune throughout the plate surface the pattern area and/or shape to study how geometry influences cell architecture and behavior
• have different micropatterns placed side-by-side (to observe them side-by-side in the same field)
*down to line widths of 3µm and dots of 5µm
They are compatible with all common HCS imaging and image analysis platforms.
Product specification
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Reference:20-950-10Size:CustomCoating:FibronectinQuantitySet of 1
Publications
Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis
26 May 2014
Keywords:
pericentrosomal Golgi ribbon organization, AKAP450 N-terminal fragments, microtubule nucleation, centrosomal activity, Golgi apparatus positioning, directional cell migration, ciliogenesis, Y-shaped micropatterned coverslips, cell movement
Spatial organization of the extracellular matrix regulates cell-cell junction positioning
24 May 2014
Keywords:
intercellular junctions, cell line, extracellular matrix
Proc. Natl. Acad. Sci. U.S.A. 2012;109(5):1506–1511.
A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells
26 May 2014
Keywords:
Live-cell microscopy, Contractility, 3D structures
J Cell Biol. 2014 Apr 14;205(1):83-96. doi: 10.1083/jcb.201311104. Epub 2014 Apr 7.
A Mechanical Checkpoint Controls Multicellular Growth through YAP/TAZ Regulation by Actin-Processing Factors
26 May 2014
Keywords:
YAP/TAZ, oncology, cell shape, cell proliferation
Cell. 2013; Aug 15 [epub ahead of print].
A method to recapitulate early embryonic spatial patterning in human embryonic stem cells.
18 July 2014
Keywords:
hESC, Assay, stem cells, Developmental Biology, Maturation, Differentiation, Gastrulation, Spacial Patterning
Nat Methods. 2014 Jun 29. doi: 10.1038/nmeth.3016
A Novel Organelle Map Framework for High-Content Cell Morphology Analysis in High Throughput
26 May 2014
Keywords:
organelle map, high content screening, high throughput screening
J Biomol Screen. 2013; Aug 16 [epub ahead of print].
A review of microfabrication and hydrogel engineering for micro-organs on chips
26 May 2014
Keywords:
tissue engineering, 3D cell culture
Biomaterials. 2013; Dec 04 [epub ahead of print].
Anisotropy of cell adhesive microenvironment governs cell internal organization and orientation of polarity
26 May 2014
Keywords:
Cell polarity, extracellular matrix, morphogenesis, cell normalization, organelle positioning
Proc. Natl. Acad. Sci. U.S.A. 2006;103(52):19771–19776.
Arl8 and SKIP act together to link lysosomes to kinesin-1
26 May 2014
Keywords:
lysosomes, kinesin
Dev. Cell. 2011;21(6):1171–1178.
Assessing Kinetics from Fixed Cells Reveals Activation of the Mitotic Entry Network at the S/G2 Transition
26 May 2014
Keywords:
cell cycle, cell division, mitosis
Molecular Cell - 27 February 2014
Carminomycin I is an apoptosis inducer that targets the Golgi complex in clear cell renal carcinoma cells
26 May 2014
Keywords:
high-throughput screening assays, clear cell renal cell carcinoma (CCRCC), Von Hippel-Lindau (VHL) tumor suppressor gene, Golgi sequestration, P-gp-mediated localization of anticancer compounds, disk and L shaped fibronectin micropattern
Cancer Res. 2011;71(1):134–142.
CD81 regulates cell migration through its association with Rac GTPase
26 May 2014
Keywords:
cell migration, tetraspanin, Rho GTPases; focal adhesions
Mol. Biol. Cell. 2012; Dec 05 [epub ahead of print].
Cell confinement controls centrosome positioning and lumen initiation during epithelial morphogenesis
26 May 2014
Keywords:
morphogenesis, cell normalization
J Cell Biol. 2012;198(6):1011-1023.
Cell distribution of stress fibres in response to the geometry of the adhesive environment
26 May 2014
Keywords:
pattern geometry, apices of cell shape, stress fibres, focal adhesions, tractional forces, cell shape, cell surface, cell adhesion, cell line
Cell Motil. Cytoskeleton. 2006;63(6):341–355
Cell shape and contractility regulate ciliogenesis in cell cycle-arrested cells
26 May 2014
Keywords:
Primary cilium, cilia, cell shape, Cell polarity
J. Cell Biol. 2010;191(2):303–312.
Cells in tight spaces: the role of cell shape in cell function
26 May 2014
Keywords:
cell shape, cell function, cell physiological phenomena, ciliogenesis
J. Cell Biol. 2010;191(2):233–236.
Chapter 7 - Methods for Analysis of Apical Lumen Trafficking Using Micropatterned 3D Systems
26 May 2014
Keywords:
3D cell culture, morphogenesis
Methods in Cell Biology.Vol Volume 118. Methods for Analysis of Golgi Complex Function. Academic Press; 2013:105–123.
Chapter 7 - Studying Mitochondria and Microtubule Localization and Dynamics in Standardized Cell Shapes
26 May 2014
Keywords:
mitochondria, microtubules, cell shape
Methods in Cell Biology. Vol 115. Academic Press; 2013:97–108.
Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation
26 May 2014
Keywords:
spindle orientation on L-patterned fibronectin-coated micropatterns, RNAi experiments on patterned coverslips, dynein-dynactin localization, Polo-like kinase 1, NuMA, LGN, RanGTP
Nat. Cell Biol. 2012;14(3):311–317.
Cortical Dynein and Asymmetric Membrane Elongation Coordinately Position the Spindle in Anaphase
26 May 2014
Keywords:
cell division, dynein, spindle orientation
Cell. 2013;154(2):391–402.
Cortical dynein is critical for proper spindle positioning in human cells
26 May 2014
Keywords:
golgi, spindle positioning, dynein
J Cell Biol. 2012;199(1):97.
Directional Persistence of Migrating Cells Requires Kif1C-Mediated Stabilization of Trailing Adhesions
26 May 2014
Keywords:
cell migration, Kif1C, transport kinesin, directional migration
Dev Cell. 2012;23(6):1153–1166.
Dynamics of Cell Shape and Forces on Micropatterned Substrates Predicted by a Cellular Potts Model
13 November 2014
Keywords:
Forces, dynamics, biophysics, modelling, cell behavior, spreading, prediction
Biophys J. 2014 Jun 3;106(11):2340-52. doi: 10.1016/j.bpj.2014.04.036.
Experimental and theoretical study of mitotic spindle orientation
26 May 2014
Keywords:
mitotic spindle orientation, cell microenvironment geometry, cortical cues, spindle pole, symmetric/ asymmetric orientation
Nature. 2007;447(7143):493–496.
FAK transduces extracellular forces that orient the mitotic spindle and control tissue morphogenesis
13 November 2014
Keywords:
mitotic spindle, morphogenesis, FAK, development, embryo, mitosis
Nature Communications 5, Article number: 5240 doi:10.1038/ncomms6240
Filamin A-Hinge region 1-EGFP : a novel tool for tracking the cellular function of filaminA in real-time
26 May 2014
Keywords:
cellular function, Filamin A, cell normalization
Plos One. 2012;7(8):e40864.
Filamin A-hinge region 1-EGFP: a novel tool for tracking the cellular functions of Filamin A in real-time
26 May 2014
Keywords:
Filamin A, cell surface, cell physiological phenomena
PLoS ONE. 2012;7(8):e40864.
Hippo Pathway Effectors Control Cardiac Progenitor Cell Fate by Acting as Dynamic Sensors of Substrate Mechanics and Nanostructure
27 May 2014
Keywords:
adult cardiac progenitor cell, substrate nanotopography, mechano-transduction; cardiac differentiation; YAP/TAZ
ACS Nano, 2014, 8 (3), pp 2033–2047 Publication Date (Web): February 2, 2014
Human Bone Marrow Mesenchymal Stem Cells Regulate Biased DNA Segregation in Response to Cell Adhesion Asymmetry
26 May 2014
Keywords:
stems cells, cell division, cell adhesion
Cell Rep. 2013;Oct 17 [epub ahead of print].
Improved visualization and quantitative analysis of drug effects using micropatterned cells
26 May 2014
Keywords:
Reference Cell, drug profiling, quantitative cell analysis, cell adhesion, cytoskeleton, drug evaluation
J Vis Exp. 2010;(46):pii: 2514.
Intercellular and extracellular mechanotransduction in cardiac myocytes
26 May 2014
Keywords:
adult cardiomyocytes, extracellular matrix, mechanical determinants, cytoskeletal organization, shape remodelling, sarcomere/myofibrillar architectural topography, cell shape-dependent functions, myopathies, myocardial infarctus, heart failure, reverse remodeling, disk-, H-, Y-, crossbow-shaped micropatterns, sarcomeric alpha-actinin.
Pflugers Arch. 2011;462(1):75–87.
Intracellular Electric Field and pH Optimize Protein Localization and Movement
26 May 2014
Keywords:
EGFR/ MAPK pathway, intracellular electric field
PLoS ONE. 2012;7(5):e36894.
Lack of a Functional VHL Gene Product Sensitizes Renal Cell Carcinoma Cells to the Apoptotic Effects of the Protein Synthesis Inhibitor Verrucarin A
26 May 2014
Keywords:
cell proliferation, apoptosis, cytotoxic stress
Neoplasia. 2012;14(8):771–777.
LNK (SH2B3) is a key regulator of integrin signaling in endothelial cells and targets α-parvin to control cell adhesion and migration
26 May 2014
Keywords:
cell migration, cell adhesion, focal adhesions
FASEB J. 2012;26(6):2592–2606.
Long-Lasting Effect of Perinatal Exposure to L-tryptophan on Circadian Clock of Primary Cell Lines Established from Male Offspring Born from Mothers Fed on Dietary Protein Restriction
26 May 2014
Keywords:
circadian clock, undernutrition, primary cell line, L-tryptophan
PLoS ONE. 2013;8(2):e56231.
MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis
26 May 2014
Keywords:
mitotic spindle orientation, H pattern, cell division, cell line, mitosis, mitotic spindle apparatus, microtubules
Nat. Cell Biol. 2011;13(9):1040–1050.
Mechanical control of epithelial lumen formation
26 May 2014
Keywords:
epithelial cells, lumen, ciliogenesis, centrosome, morphogenesis
Small GTPases. 2013;4(2).
Mechanism of the Ca2+-Dependent Interaction between S100A4 and Tail Fragments of Nonmuscle Myosin Heavy Chain IIA.
26 May 2014
Keywords:
epidermoid carcinoma A431/SIP1 cells constitutively expressing the GFP-tagged heavy chain of non-muscle myosin IIA, medium Y-shaped fibronectin micropattern
J Mol Biol. 2011;405(4-3):1004–1026.
Mechanisms of spindle positioning: cortical force generators in the limelight
26 May 2014
Keywords:
cell division, spindle, microtubules
Curr Opin Cell Biol. 2013;Aug 16 [epub ahead of print].
Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes
24 May 2014
Keywords:
tumor cells, Micropatterns, H shape, Y shape, cell division, neoplasms, cell adhesion, cell line, cell shape, Cell polarity
Genes Dev. 2008;22(16):2189–2203.
Microfluidic cell trap array for controlled positioning of single cells on adhesive micropatterns
26 May 2014
Keywords:
cell shape, Cell polarity, cell function, cell migration, single cells
Lab Chip. 2013;13(4):714–721.
Micropatterned substrates coated with neuronal adhesion molecules for high-content study of synapse formation
26 May 2014
Keywords:
neuroscience, neuron cell, cell adhesion, morphogenesis
Nat Commun. 2013;4(2252).
Micropatterning as a tool to decipher cell morphogenesis and functions
24 May 2014
Keywords:
cell microenvironment, cell architecture, engineered micropatterns, cell morphogenetic processes, micropattern shape, actin, microtubule, cell differentiation, Cell polarity, cell shape, cell proliferation
J. Cell. Sci. 2010;123(24):4201–4213.
Morphological restriction of human coronary artery endothelial cells substantially impacts global gene expression patterns
26 May 2014
Keywords:
endothelial cells, cell morphology, cytoskeleton, genomics
FEBS J. 2013; June 27 [epub ahead of print].
Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation
26 May 2014
Keywords:
immunology, Buruli, mycolactone
J Clin Invest. 2013; Mar 15 [epub ahead of print].
Myosin-Va and Dynamic Actin Oppose Microtubules to Drive Long-Range Organelle Transport
13 November 2014
Keywords:
microtubule, actin, Myosin, Melanosomes, Melanocytes, Reference Cell, Organelles, Trafficking
Curr Biol. 2014 Aug 4;24(15):1743-50. doi: 10.1016/j.cub.2014.06.019. Epub 2014 Jul 24.
Patterning Pluripotent Stem Cells at a Single Cell Level
26 May 2014
Keywords:
stem cells, vitronectin, extracellular matrix
J Biomater Tissue Eng. 2013;3(4):461–471
Phosphatidylinositol 5-phosphate regulates invasion through binding and activation of Tiam1
13 November 2014
Keywords:
Phosphoinositides, oncology, RPE-1, MEF, invasion, bacteriology, lymphoma, FRET, endosome
Nat Commun. 2014 Jun 6;5:4080. doi: 10.1038/ncomms5080.
Practical course on “Imaging infection: from single molecules to animals.”
26 May 2014
Keywords:
Fluorescence microscopy, bioluminescent imaging, High Throughput microscopy, super-resolution microscopy : TIRF, PALM/STORM, in vivo imaging, PETSCAN, CT-SCAN, FRET, live cell imaging, image analysis, photobleaching, phototoxicity
Microbes Infect. 2012; Nov 02 [epub ahead of print].
Probabilistic density maps to study global endomembrane organization
24 May 2014
Keywords:
endomembranes, small G proteins, cell line, intracellular membranes
Nat. Methods. 2010;7(7):560–566.
Rab GTPase Mediated Procollagen Trafficking in Ascorbic Acid Stimulated Osteoblasts
26 May 2014
Keywords:
osteoblasts, protein trafficking
PLoS ONE. 2012;7(9):e46265.
Reconstituting actomyosin-dependent mechanosensitive protein complexes in vitro
08 January 2015
Keywords:
mechanosensitivity, ABP, actomyosin, cytoskeleton, vinculin, talin, TIRF
Nat Protoc. 2015 Jan;10(1):75-89. doi: 10.1038/nprot.2014.200. Epub 2014 Dec 11.
Reconstitution of in vivo macrophage-tumor cell pairing and streaming motility on one-dimensional micro-patterned substrates
26 May 2014
Keywords:
cell migration, oncology
IntraVital. 2012;1(1):77-85.
Reprogramming cell shape with laser nano-patterning.
24 May 2014
Keywords:
cell shape, actin network reprogramming, dynamic cellular responses, laser nano-patterning, Cell polarity
J. Cell. Sci. 2012;125(9):2134–2140.
Role of YAP/TAZ in mechanotransduction
24 May 2014
Keywords:
mechanotransduction, mesenchymal stem cells, cell differentiation, cell line, cell shape, cytoskeleton
Nature. 2011;474(7350):179–183.
Spatial control of membrane receptor function using ligand nanocalipers
13 November 2014
Keywords:
Signaling, receptor, ligand, nanocaliper, breast cancer, membrane, ephrin-A5, invasion, presentation
Nat Methods. 2014 Aug;11(8):841-6. doi: 10.1038/nmeth.3025. Epub 2014 Jul 6.
Spindle positioning in human cells relies on proper centriole formation and on the microcephaly proteins CPAP and STIL
22 May 2014
Keywords:
microcephaly, mitotic spindle orientation
J. Cell. Sci. 2011;124(22):3884–3893.
Standardized mitochondrial analysis gives new insights into mitochondrial dynamics and OPA1 function. Int
22 May 2014
Keywords:
mitochondrial dynamics, mitochondrial disorder diagnostics, OPA1, neurodegenerative diseases, mitochondria
J. Biochem. Cell Biol. 2012;44(6):980–988.
The Bin/Amphiphysin/Rvs (BAR) Domain Protein Endophilin B2 Interacts with Plectin and Controls Perinuclear Cytoskeletal Architecture
26 May 2014
Keywords:
cytoskeleton, nucleus positioning
J Biol Chem. 2013;288(38):27619–27637.
The extracellular matrix guides the orientation of the cell division axis.
22 May 2014
Keywords:
cell fate, cell division, cell shape, cell membrane, Cell polarity, actin dynamics, average distribution of actin-binding proteins
Nat. Cell Biol. 2005;7(10):947–953.
The interaction between nesprins and sun proteins at the nuclear envelope is critical for force transmission between the nucleus and cytoskeleton.
22 May 2014
Keywords:
cell line, cytoskeleton, mechanotransduction, nuclear envelope, nucleo-cytoskeletal coupling, force transmission, LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, intracellular forces, nuclear positioning, cell polarization, muscular dystrophies, cardiomyopathies, crossbow-shaped micropatterns
J. Biol. Chem. 2011;286(30):26743–26753.
The role of RPGR in cilia formation and actin stability
22 May 2014
Keywords:
cilia, ciliogenesis, retinal dystrophy, actins, cytoskeleton, cell cycle, cell division, cell line, cell movement, cell shape, epithelial cells, eye proteins, focal adhesions, organogenesis
Hum. Mol. Genet. 2011;20(24):4840–4850
β1- and αv-class integrins cooperate to regulate myosin II during rigidity sensing of fibronectin-based microenvironments
26 May 2014
Keywords:
integrins, cell adhesion, cell contractility, cell motility, traction force
Nat Cell Biol. 2013; May 26 [epub ahead of print] .
Shipment and information
When you submit your Custom inquiry CYTOO will work with you to elaborate the detailed "Specification" document. The timing of this step depends on the complexity of your requirements. Once you have validated the final specifications in this document, the average turnaround time for production is 8 weeks.
The microplates are supplied with clear polystyrene lids and are packaged individually under inert gas.
The CYTOOplates should be stored at 4°C and used before the expiry date indicated on the packaging (6 months from the production date).
Stability is guaranteed for at least 3 months from date of shipment when stored at 4°C in its original packaging.
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