J. Cell Biol. 2011;193(5):917–933.
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CYTOOchambers Perfusion
Closed Perfusion CYTOOchambers with 1 or 2 channels are easy-to-use live cell imaging devices that were specifically designed for our CYTOOchips. The CYTOOchamber Perfusion consists of a main body (made from transparent acrylic), a bottom plate (in black anodized aluminum alloy) and a silicone gasket.
Channel dimensions can also be adapted to your precise specifications.
Advantages and key features :
- Closed type perfusion chamber
- Easy medium renewal
- Compatible with CYTOOchips
- Easy-to-use magnetic assembly
- Reusable
- One or two channels standard configurations as well as custom-made channel dimensions
- A Microscope stage adaptor is available
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CYTOOchamber Perfusion 1 channel |
Rectangular footprint; ext. dimensions 44 x 34 x 10 mm , 1 channel, customizable internal chamber volume; compatible w/ CYTOOchips |
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CYTOOchamber Perfusion 2 channels |
Rectangular footprint; ext. dimensions 44 x 34 x 10 mm, 2 channels, customizable internal chamber volume; compatible w/ CYTOOchips |
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Silicone Gaskets for CYTOOchamber Perfusion 1 channel |
Available on request |
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Silicone Gaskets for CYTOOchamber Perfusion 2 channels |
Available on request |
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Microscope plate adaptor for perfusion CYTOOchambers |
Available on request |
Publications
Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis
26 May 2014
Keywords:
pericentrosomal Golgi ribbon organization, AKAP450 N-terminal fragments, microtubule nucleation, centrosomal activity, Golgi apparatus positioning, directional cell migration, ciliogenesis, Y-shaped micropatterned coverslips, cell movement
Spatial organization of the extracellular matrix regulates cell-cell junction positioning
24 May 2014
Keywords:
intercellular junctions, cell line, extracellular matrix
Proc. Natl. Acad. Sci. U.S.A. 2012;109(5):1506–1511.
A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells
26 May 2014
Keywords:
Live-cell microscopy, Contractility, 3D structures
J Cell Biol. 2014 Apr 14;205(1):83-96. doi: 10.1083/jcb.201311104. Epub 2014 Apr 7.
A Mechanical Checkpoint Controls Multicellular Growth through YAP/TAZ Regulation by Actin-Processing Factors
26 May 2014
Keywords:
YAP/TAZ, oncology, cell shape, cell proliferation
Cell. 2013; Aug 15 [epub ahead of print].
A Novel Organelle Map Framework for High-Content Cell Morphology Analysis in High Throughput
26 May 2014
Keywords:
organelle map, high content screening, high throughput screening
J Biomol Screen. 2013; Aug 16 [epub ahead of print].
A review of microfabrication and hydrogel engineering for micro-organs on chips
26 May 2014
Keywords:
tissue engineering, 3D cell culture
Biomaterials. 2013; Dec 04 [epub ahead of print].
Anisotropy of cell adhesive microenvironment governs cell internal organization and orientation of polarity
26 May 2014
Keywords:
Cell polarity, extracellular matrix, morphogenesis, cell normalization, organelle positioning
Proc. Natl. Acad. Sci. U.S.A. 2006;103(52):19771–19776.
Arl8 and SKIP act together to link lysosomes to kinesin-1
26 May 2014
Keywords:
lysosomes, kinesin
Dev. Cell. 2011;21(6):1171–1178.
Assessing Kinetics from Fixed Cells Reveals Activation of the Mitotic Entry Network at the S/G2 Transition
26 May 2014
Keywords:
cell cycle, cell division, mitosis
Molecular Cell - 27 February 2014
Carminomycin I is an apoptosis inducer that targets the Golgi complex in clear cell renal carcinoma cells
26 May 2014
Keywords:
high-throughput screening assays, clear cell renal cell carcinoma (CCRCC), Von Hippel-Lindau (VHL) tumor suppressor gene, Golgi sequestration, P-gp-mediated localization of anticancer compounds, disk and L shaped fibronectin micropattern
Cancer Res. 2011;71(1):134–142.
CD81 regulates cell migration through its association with Rac GTPase
26 May 2014
Keywords:
cell migration, tetraspanin, Rho GTPases; focal adhesions
Mol. Biol. Cell. 2012; Dec 05 [epub ahead of print].
Cell confinement controls centrosome positioning and lumen initiation during epithelial morphogenesis
26 May 2014
Keywords:
morphogenesis, cell normalization
J Cell Biol. 2012;198(6):1011-1023.
Cell distribution of stress fibres in response to the geometry of the adhesive environment
26 May 2014
Keywords:
pattern geometry, apices of cell shape, stress fibres, focal adhesions, tractional forces, cell shape, cell surface, cell adhesion, cell line
Cell Motil. Cytoskeleton. 2006;63(6):341–355
Cell shape and contractility regulate ciliogenesis in cell cycle-arrested cells
26 May 2014
Keywords:
Primary cilium, cilia, cell shape, Cell polarity
J. Cell Biol. 2010;191(2):303–312.
Cells in tight spaces: the role of cell shape in cell function
26 May 2014
Keywords:
cell shape, cell function, cell physiological phenomena, ciliogenesis
J. Cell Biol. 2010;191(2):233–236.
Chapter 7 - Methods for Analysis of Apical Lumen Trafficking Using Micropatterned 3D Systems
26 May 2014
Keywords:
3D cell culture, morphogenesis
Methods in Cell Biology.Vol Volume 118. Methods for Analysis of Golgi Complex Function. Academic Press; 2013:105–123.
Chapter 7 - Studying Mitochondria and Microtubule Localization and Dynamics in Standardized Cell Shapes
26 May 2014
Keywords:
mitochondria, microtubules, cell shape
Methods in Cell Biology. Vol 115. Academic Press; 2013:97–108.
Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation
26 May 2014
Keywords:
spindle orientation on L-patterned fibronectin-coated micropatterns, RNAi experiments on patterned coverslips, dynein-dynactin localization, Polo-like kinase 1, NuMA, LGN, RanGTP
Nat. Cell Biol. 2012;14(3):311–317.
Cortical Dynein and Asymmetric Membrane Elongation Coordinately Position the Spindle in Anaphase
26 May 2014
Keywords:
cell division, dynein, spindle orientation
Cell. 2013;154(2):391–402.
Cortical dynein is critical for proper spindle positioning in human cells
26 May 2014
Keywords:
golgi, spindle positioning, dynein
J Cell Biol. 2012;199(1):97.
Directional Persistence of Migrating Cells Requires Kif1C-Mediated Stabilization of Trailing Adhesions
26 May 2014
Keywords:
cell migration, Kif1C, transport kinesin, directional migration
Dev Cell. 2012;23(6):1153–1166.
Experimental and theoretical study of mitotic spindle orientation
26 May 2014
Keywords:
mitotic spindle orientation, cell microenvironment geometry, cortical cues, spindle pole, symmetric/ asymmetric orientation
Nature. 2007;447(7143):493–496.
Filamin A-Hinge region 1-EGFP : a novel tool for tracking the cellular function of filaminA in real-time
26 May 2014
Keywords:
cellular function, Filamin A, cell normalization
Plos One. 2012;7(8):e40864.
Filamin A-hinge region 1-EGFP: a novel tool for tracking the cellular functions of Filamin A in real-time
26 May 2014
Keywords:
Filamin A, cell surface, cell physiological phenomena
PLoS ONE. 2012;7(8):e40864.
Human Bone Marrow Mesenchymal Stem Cells Regulate Biased DNA Segregation in Response to Cell Adhesion Asymmetry
26 May 2014
Keywords:
stems cells, cell division, cell adhesion
Cell Rep. 2013;Oct 17 [epub ahead of print].
Improved visualization and quantitative analysis of drug effects using micropatterned cells
26 May 2014
Keywords:
Reference Cell, drug profiling, quantitative cell analysis, cell adhesion, cytoskeleton, drug evaluation
J Vis Exp. 2010;(46):pii: 2514.
Intercellular and extracellular mechanotransduction in cardiac myocytes
26 May 2014
Keywords:
adult cardiomyocytes, extracellular matrix, mechanical determinants, cytoskeletal organization, shape remodelling, sarcomere/myofibrillar architectural topography, cell shape-dependent functions, myopathies, myocardial infarctus, heart failure, reverse remodeling, disk-, H-, Y-, crossbow-shaped micropatterns, sarcomeric alpha-actinin.
Pflugers Arch. 2011;462(1):75–87.
Intracellular Electric Field and pH Optimize Protein Localization and Movement
26 May 2014
Keywords:
EGFR/ MAPK pathway, intracellular electric field
PLoS ONE. 2012;7(5):e36894.
Lack of a Functional VHL Gene Product Sensitizes Renal Cell Carcinoma Cells to the Apoptotic Effects of the Protein Synthesis Inhibitor Verrucarin A
26 May 2014
Keywords:
cell proliferation, apoptosis, cytotoxic stress
Neoplasia. 2012;14(8):771–777.
LNK (SH2B3) is a key regulator of integrin signaling in endothelial cells and targets α-parvin to control cell adhesion and migration
26 May 2014
Keywords:
cell migration, cell adhesion, focal adhesions
FASEB J. 2012;26(6):2592–2606.
Long-Lasting Effect of Perinatal Exposure to L-tryptophan on Circadian Clock of Primary Cell Lines Established from Male Offspring Born from Mothers Fed on Dietary Protein Restriction
26 May 2014
Keywords:
circadian clock, undernutrition, primary cell line, L-tryptophan
PLoS ONE. 2013;8(2):e56231.
MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis
26 May 2014
Keywords:
mitotic spindle orientation, H pattern, cell division, cell line, mitosis, mitotic spindle apparatus, microtubules
Nat. Cell Biol. 2011;13(9):1040–1050.
Mechanical control of epithelial lumen formation
26 May 2014
Keywords:
epithelial cells, lumen, ciliogenesis, centrosome, morphogenesis
Small GTPases. 2013;4(2).
Mechanism of the Ca2+-Dependent Interaction between S100A4 and Tail Fragments of Nonmuscle Myosin Heavy Chain IIA.
26 May 2014
Keywords:
epidermoid carcinoma A431/SIP1 cells constitutively expressing the GFP-tagged heavy chain of non-muscle myosin IIA, medium Y-shaped fibronectin micropattern
J Mol Biol. 2011;405(4-3):1004–1026.
Mechanisms of spindle positioning: cortical force generators in the limelight
26 May 2014
Keywords:
cell division, spindle, microtubules
Curr Opin Cell Biol. 2013;Aug 16 [epub ahead of print].
Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes
24 May 2014
Keywords:
tumor cells, Micropatterns, H shape, Y shape, cell division, neoplasms, cell adhesion, cell line, cell shape, Cell polarity
Genes Dev. 2008;22(16):2189–2203.
Microfluidic cell trap array for controlled positioning of single cells on adhesive micropatterns
26 May 2014
Keywords:
cell shape, Cell polarity, cell function, cell migration, single cells
Lab Chip. 2013;13(4):714–721.
Micropatterned substrates coated with neuronal adhesion molecules for high-content study of synapse formation
26 May 2014
Keywords:
neuroscience, neuron cell, cell adhesion, morphogenesis
Nat Commun. 2013;4(2252).
Micropatterning as a tool to decipher cell morphogenesis and functions
24 May 2014
Keywords:
cell microenvironment, cell architecture, engineered micropatterns, cell morphogenetic processes, micropattern shape, actin, microtubule, cell differentiation, Cell polarity, cell shape, cell proliferation
J. Cell. Sci. 2010;123(24):4201–4213.
Morphological restriction of human coronary artery endothelial cells substantially impacts global gene expression patterns
26 May 2014
Keywords:
endothelial cells, cell morphology, cytoskeleton, genomics
FEBS J. 2013; June 27 [epub ahead of print].
Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation
26 May 2014
Keywords:
immunology, Buruli, mycolactone
J Clin Invest. 2013; Mar 15 [epub ahead of print].
Patterning Pluripotent Stem Cells at a Single Cell Level
26 May 2014
Keywords:
stem cells, vitronectin, extracellular matrix
J Biomater Tissue Eng. 2013;3(4):461–471
Practical course on “Imaging infection: from single molecules to animals.”
26 May 2014
Keywords:
Fluorescence microscopy, bioluminescent imaging, High Throughput microscopy, super-resolution microscopy : TIRF, PALM/STORM, in vivo imaging, PETSCAN, CT-SCAN, FRET, live cell imaging, image analysis, photobleaching, phototoxicity
Microbes Infect. 2012; Nov 02 [epub ahead of print].
Probabilistic density maps to study global endomembrane organization
24 May 2014
Keywords:
endomembranes, small G proteins, cell line, intracellular membranes
Nat. Methods. 2010;7(7):560–566.
Rab GTPase Mediated Procollagen Trafficking in Ascorbic Acid Stimulated Osteoblasts
26 May 2014
Keywords:
osteoblasts, protein trafficking
PLoS ONE. 2012;7(9):e46265.
Reconstitution of in vivo macrophage-tumor cell pairing and streaming motility on one-dimensional micro-patterned substrates
26 May 2014
Keywords:
cell migration, oncology
IntraVital. 2012;1(1):77-85.
Reprogramming cell shape with laser nano-patterning.
24 May 2014
Keywords:
cell shape, actin network reprogramming, dynamic cellular responses, laser nano-patterning, Cell polarity
J. Cell. Sci. 2012;125(9):2134–2140.
Role of YAP/TAZ in mechanotransduction
24 May 2014
Keywords:
mechanotransduction, mesenchymal stem cells, cell differentiation, cell line, cell shape, cytoskeleton
Nature. 2011;474(7350):179–183.
Spindle positioning in human cells relies on proper centriole formation and on the microcephaly proteins CPAP and STIL
22 May 2014
Keywords:
microcephaly, mitotic spindle orientation
J. Cell. Sci. 2011;124(22):3884–3893.
Standardized mitochondrial analysis gives new insights into mitochondrial dynamics and OPA1 function. Int
22 May 2014
Keywords:
mitochondrial dynamics, mitochondrial disorder diagnostics, OPA1, neurodegenerative diseases, mitochondria
J. Biochem. Cell Biol. 2012;44(6):980–988.
The Bin/Amphiphysin/Rvs (BAR) Domain Protein Endophilin B2 Interacts with Plectin and Controls Perinuclear Cytoskeletal Architecture
26 May 2014
Keywords:
cytoskeleton, nucleus positioning
J Biol Chem. 2013;288(38):27619–27637.
The extracellular matrix guides the orientation of the cell division axis.
22 May 2014
Keywords:
cell fate, cell division, cell shape, cell membrane, Cell polarity, actin dynamics, average distribution of actin-binding proteins
Nat. Cell Biol. 2005;7(10):947–953.
The interaction between nesprins and sun proteins at the nuclear envelope is critical for force transmission between the nucleus and cytoskeleton.
22 May 2014
Keywords:
cell line, cytoskeleton, mechanotransduction, nuclear envelope, nucleo-cytoskeletal coupling, force transmission, LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, intracellular forces, nuclear positioning, cell polarization, muscular dystrophies, cardiomyopathies, crossbow-shaped micropatterns
J. Biol. Chem. 2011;286(30):26743–26753.
The role of RPGR in cilia formation and actin stability
22 May 2014
Keywords:
cilia, ciliogenesis, retinal dystrophy, actins, cytoskeleton, cell cycle, cell division, cell line, cell movement, cell shape, epithelial cells, eye proteins, focal adhesions, organogenesis
Hum. Mol. Genet. 2011;20(24):4840–4850
β1- and αv-class integrins cooperate to regulate myosin II during rigidity sensing of fibronectin-based microenvironments
26 May 2014
Keywords:
integrins, cell adhesion, cell contractility, cell motility, traction force
Nat Cell Biol. 2013; May 26 [epub ahead of print] .
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